2016 Fiscal Year Final Research Report
Simultaneous extraction and analyses of RNA and DNA from single cells
| Project/Area Number |
26630052
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fluid engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
Santiago Juan G. Stanford University, 教授
NISHIKII Hidekazu Stanford University, Visiting Scholar
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | マイクロ流路 / 電気泳動 / 一細胞 / RNA / DNA / シークエンシング / 電気穿孔 / 前処理 |
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| Outline of Final Research Achievements |
In this project, we have developed a novel extraction technique of RNA and DNA from single cells with electrical lysis and electrokinetics and demonstrated it with integrated analyses on RNA and DNA. The technique leverages a microfluidic system to enable selective lysis of cytoplasmic membrane in less than 200 ms and completes extraction and separation between cytoplasmic RNA and a nucleus in less than one minute. The extracted RNA and DNA molecules showed high quality to perform high-throughput sequencing analyses. We also demonstrated our techniques with integrated nuclear and cytoplasmic RNA sequencing analyses and proposed a new strategy to study RNA transport at single cell resolution.
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| Free Research Field |
流体工学
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