2015 Fiscal Year Final Research Report
Hyper-miniaturization of fluorescence multisensor for pathogens with high sensitivity by evanescent light
| Project/Area Number |
26630243
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Hokkaido University |
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Principal Investigator |
Satoh Hisashi 北海道大学, 工学(系)研究科(研究院), 准教授 (80326636)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Satoshi 北海道大学, 大学院工学研究院, 助教 (10612674)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | バイオセンサ |
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| Outline of Final Research Achievements |
Gold thin film was immobilized on a glass plate. DSP molecules, which served as self-assembled monolayer, were immobilized on it. Anti-E. coli O157 antibody was immobilized on it. The glass plate was called as a sensor chip. The electrical signal was measured through a redox couple [Fe(CN)6]3－/4－ by cyclic voltammetric analysis. The lower detection limit was 10 cell/mL.
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| Free Research Field |
水環境工学
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