Rapid detection of infectious enteric viruses in water using cellular responses to virus infection

2015 Fiscal Year Final Research Report

• Project/Area Number: 26630244
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Tohoku University
• Principal Investigator: Omura Tatsuo 東北大学, 未来科学技術共同研究センター, 教授 (30111248)
• Co-Investigator(Kenkyū-buntansha): DAISUKE SANO 北海道大学, 大学院工学研究院, 准教授 (80550368)
• Co-Investigator(Renkei-kenkyūsha): YOSHIFUMI MASAGO 国際連合大学, サステイナビリティ高等研究所, 研究員 (50507895)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 腸管系ウイルス / 細胞応答 / 迅速検出

Outline of Final Research Achievements

This study aimed to identify candidate genes for detecting infectious viruses when concentrated water samples were inoculated to cultivated human cell lines. A vaccine strain of poliovirus type 1 (PV1) was used as a test virus, and the INT407 cell line derived from human intestinal epithelial cells was used as a host cell line. The expression level of KCNJ4 and SCN7A genes were monitored in the post-infection of PV1. When PV1 was inoculated at multiplicity of infection (MOI) = 1, the expression levels of KCNJ4 and SCN7A genes were significantly increased compared to those in mock cells. These results indicate that these genes of ion-chancel proteins are available as cellular genetic markers for detecting the presence of infectious viruses in water samples.

Free Research Field

土木環境システム