Identification of mushroom gene involving in absorption and concentration of Cs, and development of innovative technology by the exploitation

2016 Fiscal Year Final Research Report

• Project/Area Number: 26630249
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Kagoshima National College of Technology
• Principal Investigator: Masahito Yamauchi 鹿児島工業高等専門学校, 都市環境デザイン工学科, 教授 (40239843)
• Co-Investigator(Renkei-kenkyūsha): YAMAGUCHI Takashi 長岡技術科学大学, 工学研究科, 教授 (10280447)
• Research Collaborator: YAGI Fumio 鹿児島大学, 名誉教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: きのこ / セシウム / カリウム / カリウムトランスポーター

Outline of Final Research Achievements

Primers of K transporters (TRKH) 1~4 and formyltetrahydrofolate synthetase with Cs binding site were designed using genome information on Pleurotus osteatus. PCR with extracted DNA and primers revealed TRKH 2
and 3 could be amplified. Real time RT-PCR on TRKH2 showed diazoxide (previously confirmed as activator for Cs intake) increased the gene expression, suggesting that this transporter was involved in the Cs intake.

Free Research Field

環境工学, 廃棄物工学