Enrichment and isolation of nitrous oxide reducing bacteria by a gas-permeable membrane device

2015 Fiscal Year Final Research Report

• Project/Area Number: 26630420
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Terada Akihiko 東京農工大学, 工学(系)研究科(研究院), 准教授 (30434327)
• Co-Investigator(Renkei-kenkyūsha): HORI Tomoyuki 国立研究開発法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (20509533)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: N2O還元細菌 / 亜酸化窒素（N2O） / ガス透過膜 / 細菌 / 単離 / 動力学的解析 / 集積化 / nosZ

Outline of Final Research Achievements

A novel enrichment device by a gas-permeable membrane was developed to acquire highly-effective nitrous oxide (N2O)-reducing bacteria able to reduce N2O, highly-potent greenhouse gas, into nitrogen gas. Long-term operation of the enrichment device revealed that selective enrichment of nosZ clade II type N2O-reducing bacteria, newly discovered N2O reducers, is feasible by the device. Especially, species in the genus Dechloromonas of the family Rhodocyclaceae was found predominant in the device. Furthermore, the enriched species in the device was successfully and effectively isolated. The isolated species is phylogenetically analogous to the predominant species enriched in the device. Biokinetic analysis illuminated that the isolated Dechloromonas species, belonging to nosZ clade II type, has higher affinity for N2O than the previously found N2O reducing bacteria harboring nosZ clade I.

Free Research Field

環境バイオテクノロジー