2015 Fiscal Year Final Research Report
Development of a novel immunoassay system using NIR luminescent glass
| Project/Area Number |
26630425
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Nagoya University |
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Principal Investigator |
Nakano Hideo 名古屋大学, 生命農学研究科, 教授 (00237348)
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| Co-Investigator(Kenkyū-buntansha) |
渕 真悟 青山学院大学, 理工学部, 准教授 (60432241)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 免疫測定 / 近赤外蛍光 / ルシフェラーゼ / 抗体 |
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| Outline of Final Research Achievements |
We have developed a novel and rapid immunoassay system, named BINGO (Bioluminescent Interference Gathering Optical) assay, without any separation steps. It employs luciferase-antibody complex (LucAb). Initially, antibody-luciferase are uniformly dispersed in the solution. The luminescent excited by the luc was detected by photo-multiplier tube (PMT) for near-infrared light. Localization of antigen-antibody complex to the fluorescent glass increased the PMT signal. Then another system without the NIR-fluorescent glass was also tried. A PMT detector for visible light was equipped under the bottom, so the luminesce by Luc was directly detected. The principle was initially demonstrated in a model system, and it also successively detected E. coli O157 (toxin-defective) in a 30 min.
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| Free Research Field |
生物工学
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