Total culture fingerprint evaluation method for standardization of multi-potent stem cell culture

2015 Fiscal Year Final Research Report

• Project/Area Number: 26630427
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: KATO RYUJI 名古屋大学, 創薬科学研究科, 准教授 (50377884)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 再生医療 / 標準化 / 幹細胞 / 細胞培養工程 / 画像処理 / 数値化 / iPS細胞 / 細胞品質

Outline of Final Research Achievements

Pluripotent stem cells, such as iPS cells, require highly trained culture skills, therefore the present cell culture production process greatly rely on experts' skills and experiences. To widely distribute the stem cell technology, the quantitative understanding and standardization is essential. In this study, we have developed an image-based profiling method, "Total culture fingerprint", to record and monitor the quality changes during their culture. By our method, we have found that the stress levels and effects on disturbance of iPS cellular quality can be monitored and modeled by real-time analysis of their colony morphologies, which are known to be an indicator of their quality change.

Free Research Field

医療支援工学