Development of efficient protein production system by photo-driven ATP regenerating vesicle

2014 Fiscal Year Final Research Report

• Project/Area Number: 26630430
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: HARA Kiyotaka 神戸大学, 自然科学系先端融合研究環重点研究部, 准教授 (40434378)
• Co-Investigator(Kenkyū-buntansha): IMAMURA Hiromi 京都大学, 白眉センター, 准教授 (20422545) ; WATANABE Yohei 甲南大学, 理工学部, 准教授 (40411839) ; SUNO Ryoji 京都大学, 医学(系)研究科(研究院), 研究員 (60447521)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: ATP / Protein / Probe / Photo / Chaperone / Structure

Outline of Final Research Achievements

Conventional cell-free protein synthesis system has fundamental problem in efficiency, sustainability and cost due to the shortage of ATP as an energy source. We aimed to resolve this problem by photosynthetic ATP supply using ATP biosensor and ATP photo-synthetic vesicle. As the result, we could not identify the condition in which the photo-synthetic vesicle, cell-free protein synthesis system and chaperon work together, although we succeeded to improve the sensitivity of the ATP biosensor. On the other hand, GPCR was synthesized using wheat cell-free protein synthesis system as a model of protein.

Free Research Field

Bioenergy