Development of a novel chloride probe aiming at detecting inhibitory synaptic inputs

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640002
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Saitama University
• Principal Investigator: OHKURA Masamichi 埼玉大学, 理工学研究科, 准教授 (70369172)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 神経科学 / 神経活動 / 抑制性シナプス / クロライドプローブ / 蛍光イメージング

Outline of Final Research Achievements

Increases in intracellular chloride ion concentrations of neurons following inhibitory postsynaptic potentials (IPSPs) have not been visualized to date. In this study, aiming at detecting IPSPs, we developed a genetically encoded chloride probe with high responsivity. The probe showed a fairly large fluorescence change in response to chloride in vitro. Then we expressed the probe in neuronal cells and assessed its functionalities, focusing on responses to inhibitory inputs.

Free Research Field

分子生理学