2016 Fiscal Year Final Research Report
Developmental epigenetic regulation for neuronal diversity
| Project/Area Number |
26640012
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | University of Yamanashi (2015-2016)
Osaka University (2014) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 神経細胞 / エピジェネティクス / 多様性 / 神経回路 / iPS細胞 |
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| Outline of Final Research Achievements |
By conducting DNA methylation analysis from the embryo to the postnatal brain, establishment of the iPS cell line from the embryonic lethal mouse and generation of the chimeric mouse, and gene expression analysis and morphological analysis at the single neuron level, we revealed that differential DNA methylation dependent on DNA methylation enzyme Dnmt3b regulates the stochastic expression of the clustered protocadherin genes in Purkinje cells and that it is involved in pattern formation of dendrites. In addition, as a collaborative research, we found that the epigenetic regulation at the developmental stage is also involved in the formation of reciprocal synaptic connections dependent on the cell lineage of the cerebral cortex by performing double whole cell recording in the brain of chimeric mice.
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| Free Research Field |
神経科学、分子生物学
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