The mechanism of the cerebral cortical formation by genome replacement

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640028
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: OKADO Haruo 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, プロジェクトリーダー (60221842)
• Co-Investigator(Kenkyū-buntansha): HIRAI Shinobu 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (00625189) ; HIRAI Sayaka 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 研究員 (80606434)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 脳形成 / RP58 / 大脳皮質 / OSVZ / ゲノム置換

Outline of Final Research Achievements

We generated the transgenic mice with human Bac clone containing RP58 gene ( 204 kbps). By mating them with RP58 hero mice, we generated the mice with human Bac of RP58 gene lacking mouse RP58. These mice show severe motor uncontrolled, suggesting that the function of cerebellum is impaired. Therefore, we tried to generate the mice lacking mouse RP58 only in cereberal cortex with RP58 flox mice and EmxCre mice. We generated the five strains of mice with RP58 negative, but human RP58 positive in the cereberal cortex. We are analyzing behavior of the mice, and plan to perform histological analysis.

Free Research Field

神経生物