2015 Fiscal Year Final Research Report
Analyses of dynamic movement of intracellular structures underlying neuronal migration in the developing brain
| Project/Area Number |
26640040
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Chubu University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 細胞移動 / ニューロン / 微小管 / アクチン |
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| Outline of Final Research Achievements |
Neuronal migration is an integrative process involving leading process formation, nucleokinesis, and rear retraction, all of which depend on the function of cytoskeletons. In this study, live imaging of microtubule (MT) and actin cytoskeletons in migrating neocortical neurons in slice culture was performed. In migrating neurons, two types of nucleokinesis were observed. The first one is the nucleus-centrosome (N-C) coupling type of nucleokinesis. Centrosome proceeded into the leading process prior to the N-C coupling type of nucleokinesis. Rear accumulation of F-actin was not necessarily observed during the N-C coupling type of nucleokinesis. The second type of nucleokinesis is the N-C inversion during which translocating nucleus overtake the centrosome. Rear accumulations of F-actin were observed when the N-C inversion occurred. These data suggest that the actomyosin system underlies the N-C inversion type of nucleokinesis during which the MT-dynein system could not pull the nucleus.
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| Free Research Field |
細胞生物学
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