Development of an analysis system of infrared spectroscopy for membrane proteins under existence of membrane potential

2016 Fiscal Year Final Research Report

• Project/Area Number: 26640047
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Institute for Molecular Science
• Principal Investigator: FURUTANI Yuji 分子科学研究所, 生命・錯体分子科学研究領域, 准教授 (80432285)
• Research Collaborator: TAKADA Noriko 分子科学研究所, 装置開発室 ; AOYAMA Masaki 分子科学研究所, 装置開発室 ; MIZUTANI Nobuo 分子科学研究所, 装置開発室 ; KONDO Takuhiko 分子科学研究所, 装置開発室
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 膜タンパク質 / 膜電位 / 赤外分光法 / イオンチャネル

Outline of Final Research Achievements

Effect of membrane potential on the structure and dynamics of membrane proteins must be important for elucidating their molecular mechanisms such as generation of action potential in a nerve cell. In this study, we aimed to develop a new technique of infrared spectroscopy for analyzing changes in a protein structure and its internal hydrogen bonds under the membrane potential. Surface-enhancement of infrared absorption and reconstitution of membrane proteins have been examined. We obtained several results useful for conducting infrared spectroscopy on membrane proteins under existence of the membrane potential in future.

Free Research Field

生物物理学