2015 Fiscal Year Final Research Report
Development of protein knockdown method in zygote
| Project/Area Number |
26640063
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | タンパク質ノックダウン |
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| Outline of Final Research Achievements |
In this study, we try to develop a novel method for protein knockdown. We produced chimeric protein consisting of lysosome binding peptide and protein A. When this fusion proteins were microinjected to zygote with EGFP mRNA and anti-EGFP antibody, EGFP proteins were efficiently degraded. Next, we examined whether this method are applicable to endogenous Stella proteins, a maternal proteins localized in nucleus. However, Stella proteins were not degraded after microinjected with a fusion proteins and anti-Stella antibody. These data suggested that this method is applicable to cytoplasmic proteins, but not to nuclear proteins.
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| Free Research Field |
生殖細胞
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