Standardization of genome-wide absolute and accurate counting of RNA molecules

2014 Fiscal Year Final Research Report

• Project/Area Number: 26640119
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SHIROGUCHI Katsuyuki 独立行政法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (00454059)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: 高精度定量 / デジタル定量 / バーコーディング / ゲノムワイド / シークエンサ

Outline of Final Research Achievements

We have previously developed the molecular barcoding method which enables one to perform accurate digital counting of the copy number of nucleic acid molecules genome-wide using next generation sequencer. This time, we developed it further, for example, by optimizing the sequence of molecular barcode, and succeeded in obtaining high dynamic range with less cost. These results may push to make our molecular barcoding method world-standard.

Free Research Field

生物物理学　定量オミクス