Technical development of single-cell multi-omics

2016 Fiscal Year Final Research Report

• Project/Area Number: 26640120
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sasagawa Yohei 国立研究開発法人理化学研究所, 情報基盤センター, 上級センター研究員 (10404344)
• Co-Investigator(Renkei-kenkyūsha): Danno Hiroki 国立研究開発法人理化学研究所, 情報基盤センター, センター研究員 (20610439)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 1細胞RNA-seq / 1細胞エピゲノムシーケンス

Outline of Final Research Achievements

In this study, we tried to develop technology for simultaneous detection of epigenome and transcriptome from one cell. We separated cytosol and nucleus from a single-cell using pipet tip. We tried to use nucleus for detection of epigenome. Moreover, we succeeded to develop single-cell RNA-seq method, which detect non-poly-A RNA including nascent RNA. Moreover, we tried to develop cell-barcoding method using random primer for high-throughput detection of RNA.

Free Research Field

バイオテクノロジー