Molecular analysis of the differentially expressed miRNA related to progression of Alzheimer's disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 26640122
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Niigata University
• Principal Investigator: Kuwano Ryozo 新潟大学, 脳研究所, フェロー (20111734)
• Co-Investigator(Renkei-kenkyūsha): MIYASHITA Akinori 新潟大学, 脳研究所, 助教 (60323995) ; NAKAYA Akihiro 新潟大学, 研究推進機構, 准教授 (60301149)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: アルツハイマー病 / miRNA / 凍結ヒト脳

Outline of Final Research Achievements

Alzheimer’s disease (AD) is progressive neurodegenerative disorder characterized by two major pathological changes in expansion of senile plaques (SP) and neurofibrillary tangles (NFT) in the brain. Autopsy brains were diagnosed based on the degree of these two pathological findings defined by the Braak stage (SP: 0, A, B, and C, and NFT:0, I, II, III, IV, V and VI). We analyzed non-coding small RNA, especially miRNA, in the exosome as a candidate molecule of propagation of pathological lesion. Total RNA was isolated from three brain regions (entorhinal cortex, temporal cortex and frontal cortex) at several Braak stages. Over 20,000,000 single reads in each sample was determined by the next generation sequencing and statistically analyzed. We found differentially expressed miRNAs in the course of expansion of pathological lesion in each brain region.

Free Research Field

ゲノム医科学