2016 Fiscal Year Final Research Report
Culture and Freeze-Stock of Normal Cells from Hair Roots of Mammals
| Project/Area Number |
26640139
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservation of biological resources
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| Research Institution | Fukuyama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 毛根 / 哺乳動物 / 細胞培養 / 凍結保存 / 初期化 / エピソーマルベクター / 分化誘導 / ノアの箱舟 |
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| Outline of Final Research Achievements |
Using the eyebrow hair roots that could be easily removed by 7 adult volunteers themselves under informed consent, fibroblast-like normal cells were obtained with regardless of age or gender. By the same method, normal cells of other 5 species were cultured and stocked. To obtain normal cells as bio-resource from whisker or body hair of other mammals, we examined mouse, djungarian hamster and Japanese field mouse (Apodemus speciosus), syrian hamster and four toed hedgehog as model animals, then most of cultured cells were fibroblastic shape, but some morphological variation were observed. We also clarified the most suitable condition to obtain normal cells of these mammals. We cultured and freeze-stocked normal cells by a simple and minimally invasive way from the hair root of 6 species of mammals. These stocked normal cells will be deposited to the cell bank as valuable bio-resources.
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| Free Research Field |
動物細胞工学
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