2015 Fiscal Year Final Research Report
Mechanisms and functions of i-RNase activity
| Project/Area Number |
26650006
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University |
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Principal Investigator |
Kuraoka Isao 大阪大学, 基礎工学研究科, 准教授 (60335396)
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| Co-Investigator(Renkei-kenkyūsha) |
Iwai Shigenori 大阪大学, 大学院基礎工学研究科, 教授 (10168544)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | RNA / 損傷 |
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| Outline of Final Research Achievements |
Deamination of adenine occurs in DNA, RNA, and their precursors. The generated deaminated products are potentially mutagenic because of their structural similarity to natural bases. We found that human endonuclease V (i-RNase) recognizes and cleaves inosine-containing RNA.
In this study, to figure out the characters of the endonuclease activity, a series of RNA/DNA substrates was employed. Our data indicated that this enzyme is involved in RNA repair.
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| Free Research Field |
分子生物学
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