Whole genome epigenetic analysis during transdifferentiation

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650008
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: Maki Nobuyasu 大阪大学, たんぱく質研究所, 准教授 (90342849)
• Co-Investigator(Renkei-kenkyūsha): FUKAGAWA Tatsuo 大阪大学, 生命機能研究科, 教授 (60321600) ; HORI Tetsuya 大阪大学, 生命機能研究科, 准教授 (70550078)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 脱分化 / 分化転換 / 胚網膜色素上皮細胞 / エピジェネティクス

Outline of Final Research Achievements

In general it is thought that terminally differentiated cells never change into other type of cells. However, in some species cellular transdifferentiation occur. In retinal pigmented epithelial cells (RPE) in chick embryos undergo dedifferentiation and then transdifferentiate into lens cells. In this study epigenetics change during dedifferentiation of RPE was analyzed and the following characteristic features are revealed, 1) dynamic change of histone H3K3me3, 2) regulation of epigenetic mark is gene-selective rather than non-selective, and 3) the changes of each epigenetic marks are correlated.

Free Research Field

再生・発生