Developing strategy for uni-directional insertion of single transmembrane protein to liposome

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650015
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Sato Takeshi 大阪大学, たんぱく質研究所, 講師 (90403013)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 膜タンパク質 / 再構成

Outline of Final Research Achievements

Structural studies on the transmembrane region of membrane protein should be done in lipid bilayers. Direction of the transmembrane peptide insertion to the membrane often provides a problem. In this project, uni-directional insertion of a transmembrane peptide to liposome was examined. The peptide was designed to have a cell penetrating sequence in the C-terminus of the transmembrane sequence. This peptide was found to exist on the surface of the liposome. However, a longer 20-30 min of incubation, the peptide passed through the membrane. Additional techniques are required to achieve the goal. Another trial for obtaining better condtions for the reconstitution has been successful. The protocoal enabled more rapid reconstitution than the general ones that have been used.

Free Research Field

構造生物化学