Development of new cell field structure technology to elucidate the basic mechanism of eukaryotes

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650016
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Institute of Physical and Chemical Research (2015-2016); Osaka University (2014)
• Principal Investigator: Iwane Atsuko 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (30252638)
• Research Collaborator: ICHINOSE Takako 大阪大学, 生命機能研究科, 招へい研究員 (40776902) ; NAGAI Rina 国立研究開発法人理化学研究所, 生命システム研究センター, テクニカルスタッフ (60392049) ; OHTA Keisuke 国立研究開発法人理化学研究所, 生命システム研究センター, 客員主管研究員 (00258401)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: FIB-SEM / 超微細構造 / 細胞分裂 / シゾン / 三次元再構築 / ダイナミクス

Outline of Final Research Achievements

Microstructural analysis in cells and tissues has been pursued by TEM observation of ultrathin sections prepared by chemical fixation-staining. In recent years As FIB-SEM contributing to the surface processing of metals and ceramics has been used in recent years, we have developed new technologies for application for a 3D-structural analysis of biological sample using its system. We chose " Cyanidioschyzon merolae " which has a minimal simple structure as a eukaryote as a model organism, representative exists cell organelle one by one, and according to the organelles and target molecules linked to the cell division process. We clarified the ultrastructural changes of during the cell mitosis at a whole cell level. By referring to the dynamics by optical microscope, we created a 3D structural model adapted to the time series of cell division and clarified the interaction and shape change among organelles.

Free Research Field

生物物理学、分子生物学