Protein NMR method developed for macromolecular complex

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650018
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Kojima Chojiro 大阪大学, たんぱく質研究所, 准教授 (50333563)
• Co-Investigator(Renkei-kenkyūsha): FUJIWARA Toshimichi 大阪大学, 蛋白質研究所, 教授 (20242381) ; MATSUKI Yoh 大阪大学, 蛋白質研究所, 助教 (70551498) ; SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 助教 (70635698) ; FURUITA Kyoko 大阪大学, 蛋白質研究所, 研究員 (30727665)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: NMR / 蛋白質 / 立体構造

Outline of Final Research Achievements

Protein structure can be determined by solution NMR precisely, however, molecular weight is limited to below 100kDa. In this study, NMR method has been developed for protein structure analysis, especially for very big protein beyond 100kDa. Here, double-acquisition method was developed to acquire twice data within 1 scan. This double-acquisition method was combined with 13C direct detection method, and optimized for macromolecular complex. Using these advanced methods, about 70% signals of florigen receptor are assigned.

Free Research Field

構造生命科学