The development of method for the isolation of autophagosomes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650019
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: Hasegawa Junya 大阪大学, 生命機能研究科, 助教 (00533788)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: オートファジー / 脂質

Outline of Final Research Achievements

Autophagy is a bulk degradation system which is mediated by a unique organelle "autophagosome". It has been few reports on the lipid component of the autophagosome membrane. We have previously developed the unique method for the isolation of autophagosomes using latex beads. Thus, we set out to purify the autophagosomes to clarify the lipid component. We found the suitable condition to collect the high amounts and high purity of autophagosomes. Under the condition, we tried MS analysis, but unfortunately we could not detect any peak of lipids in MS. Hereafter, we will find the more suitable condition, and perform the analysis of lipid component of autophagosomes.

Free Research Field

分子細胞生物学