2015 Fiscal Year Final Research Report
Attempts to obtain electrons from water molecules by realizing the reversed reaction of enzymatic process
| Project/Area Number |
26650031
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | マルチ銅オキシダーゼ / 逆反応 / 酸素４電子還元 / 水の酸化 |
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| Outline of Final Research Achievements |
Multicopepr oxidase is a class of enzymes to perform the four-electron reduction of dioxygen at the trinuclear copper center by utilizing electrons from substrates extracted at the type I copper center. The aim of this study is to realize the reverse reaction of this four-electron reduction of dioxygen. Our strategy to realize this attempt was to shift the oxidation-reduction potential of the type I copper center towards positive direction by performing mutations at the ligands to type I copper and/or amino acids located in the outer coordination spheres. Mutations on CueO and bilirubin oxidase were succeeded but we could not realize the reverse reaction to form dioxygen even utilizing a variety of electrode materials. We are trying to realize our aim by using other multicopper oxidases.
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| Free Research Field |
生物化学
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