2015 Fiscal Year Final Research Report
Characterization of exoxome membrane as cancer progressive markers
| Project/Area Number |
26650038
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
Fukami Kiyoko 東京薬科大学, 生命科学部, 教授 (40181242)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | エキソソーム / がん悪性化 / イノシトールリン脂質 |
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| Outline of Final Research Achievements |
Lipid small vesicle exosomes have been reported to be involved in cancer progression such as metastasis and drug resistance. We hypothesized that various kinds of phosphoinositides on exosome from cancers define the characters of exosome.
We confirmed the existence of phosphoinositides on exosome by using phosphoinositides-binding probes and mass analysis. We also examined the effect of B-Raf inhibitor Vemurafenib-treatment on exosome properties from melanoma cells. With treatment of Vemurafenib, the expression of exosome marker CD63 was dramatically increased.
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| Free Research Field |
病態医科学
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