Development of scanning optical microscope capable of the excitation-emission spectral measurement

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650043
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: Shibabta Yutaka 東北大学, 理学(系)研究科(研究院), 准教授 (20300832)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 顕微分光 / 励起スペクトル

Outline of Final Research Achievements

Although the fluorescence microscopy has been applied to obtain the fluorescence spectra of a sample, it has not been applied to obtain the excitation spectra. A measurement of an excitation spectrum takes more time than that of an emission spectrum because it requires scanning of the excitation wavelength. I used the photonic-crystal fiber to convert the femto-second pulsed laser light to a white light for the excitation. I developed an optical design in which the excitation laser spots with different wavelengths are focused to different positions of the sample. This optical design enable me to realize a microscope capable of the measurement of both excitation and emissino spectra.

Free Research Field

光生物物理化学