2015 Fiscal Year Final Research Report
Medicinal application of intrinsically disordered proteins of unknown function
| Project/Area Number |
26650048
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 凍結保護 / バイオ医薬品添加剤 / コールドチェイン / 分子盾効果 / 天然変性タンパク質 |
|---|
| Outline of Final Research Achievements |
Intrinsically disordered proteins (IDPs), polypeptides that lack a stable compact structure, are known to exert the two major molecular functions, “fly-casting mechanism” and “coupled folding and binding”. Plant stress-responsible protein dehydrins and silkworm sericins are the examples of IDPs. These exert cryoprotective activity against enzymes and cultivated cells, respectively. If IDP’s molecular functions are originated from their physical properties, similar functions may also exist in human-genome derived IDPs.
Based on the concept, we started to screen several human IDPs using cryoprotective activity against the model enzyme lactose dehydrogenase as an index. Finally, we succeeded in finding a 20 aa IDP peptide that showed cryoprotective activity against NHDF cell.
|
| Free Research Field |
物理系薬学
|
