Production of bifunctional recognition biomolecules for a novel crystallization method

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650051
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: University of Hyogo
• Principal Investigator: Shibata Naoki 兵庫県立大学, 生命理学研究科, 准教授 (30295753)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 構造生物学 / X線構造解析 / 分子認識

Outline of Final Research Achievements

This research is aimed at creating non-natural interaction between proteins based on genetically modified natural proteins carrying random mutations. Initially we used artificial pentapeptide repeat proteins (PRP) for templates. However, RNA/DNA of PRP, recovered from displayed complex including template RNA/DNA and expressed protein, expresses almost insoluble proteins. Next we tried new templates, E. coli YncE and DIX. The former still yielded insoluble proteins but the latter produced relatively highly soluble ones. The selected DIX proteins after CIS-display generally showed higher affinity compared to the wild-type.

Free Research Field

構造生物学