Visualization of biological phenomena by the second harmonic generation imaging using the next generation dye.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650052
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Keio University
• Principal Investigator: Nuriya Mutsuo 慶應義塾大学, 医学部, 講師 (60453544)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 光第二高調波発生 / ２光子顕微鏡 / SHG / イメージング / アストロサイト

Outline of Final Research Achievements

One of the two-photon imaging techniques, called the second harmonic generation (SHG) imaging, can visualize unique biological phenomena because of its specific requirements for signal generation. In this study, we attempted to develop an SHG-specific dye to overcome the limitations of SHG imaging associated with uses of currently available general fluorescent dyes. We have successfully developed a non-fluorescent SHG specific dye, Ap3, and realized pure SHG imaging and true multimodal two-photon imaging using this dye. This SHG-specific dye should greatly facilitate the applications of SHG imaging in biological researches.

Free Research Field

神経科学