Development of a ratiometric fluorescent thermosensor sheet for non-invasive temperature mapping at single-cell level

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650053
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Jikei University School of Medicine (2015-2016); Waseda University (2014)
• Principal Investigator: OYAMA Kotaro 東京慈恵会医科大学, 医学部, 特別研究員 (70632131)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 温度計シート / 蛍光イメージング / 温度測定

Outline of Final Research Achievements

We developed fluorescent thermosensor sheets for non-invasive temperature mapping at single-cell level. Thermo-sensitive fluorescent dyes were spin-coated on a culture dish. The fluorescence intensity responded to changes in temperature. Human cells (HeLa, HEK293), rat neonatal cardiomyocytes, or rat hippocampal neurons were cultured on the fluorescent thermosensor sheets. By using the thermosensor sheets, we found novel temperature-sensing systems in living cells: Ca2+ burst in WI-38 cells induced by a microscopic heat pulse, neurite outgrowth triggered by microheating, and hyperthermal sarcomeric oscillations (HSOs）in cardiomyocytes.

Free Research Field

生物物理