Exploring mechanism of gene expression noise in eukaryotic cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650055
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Taniguchi Yuichi 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90556276)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: １分子・１細胞生物学 / 生物物理学 / システム生物学 / 超高感度顕微鏡技術 / 微細加工技術 / 生命反応の物理

Outline of Final Research Achievements

Gene expression is a stochastic process. Due to this, gene expression levels in single cells are inherently heterogeneous even among a genetically identical cell population. In this work, we challenged to develop a method to visualize single-molecule mRNA and protein productions in single eukaryotic cells. The results suggest that the protein translation process is a major source to regulate gene expression noise.

Free Research Field

生物物理学