The development of the method for driving ion-driven biological rotary motor with optical illumination

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650057
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Hamamatsu Photonics K.K.
• Principal Investigator: Kimura Yuji 浜松ホトニクス株式会社, 中央研究所, 専任部員 (90713371)
• Research Collaborator: KAZAMI Sayaka 浜松ホトニクス株式会社, 中央研究所, 研究員 (80727991)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 生体膜 / イオン駆動型分子モーター / 1分子計測（SMD） / マイクロ・ナノデバイス / ナノバイオ

Outline of Final Research Achievements

The purpose of this research is to drive FoF1-ATP synthase with optical illumination. For this purpose, 1) we developed an evaluation and manipulation system for FoF1-ATP synthase by using microfluidics device. The device enabled us to image proton pumping activity of respiratory chain complex I and FoF1-ATP synthase embedded on a membrane of a large-sized protoplast from E-coli, in real time. 2) To drive the ATP-synthase activity, the membrane of the large-sized E-coli protoplast was illuminated with evanescent field. The synthesized ATP was monitored with luciferin/luciferase system. In the observation period (~10 min), however, the activity was not detected. It suggests more detailed analyses on electro-chemical potential are needed.

Free Research Field

生物物理学