Direct reprograming to neutrophil-like cells by the compound 2057 that induces lobulated nuclei

2014 Fiscal Year Final Research Report

• Project/Area Number: 26650062
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kumamoto University
• Principal Investigator: TANI Tokio 熊本大学, 自然科学研究科, 教授 (80197516)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Keywords: 分葉核 / 好中球 / 化合物 / チューブリン / ラミン

Outline of Final Research Achievements

We identified the compound 2057 that induced the formation of the lobulated nuclei, a typical morphology of neutrophil nuclei, in HeLa cells. In this study, we tested the possibility that the compound 2057 triggers the direct reprograming of HeLa cells to neutrophil-like cells. After the treatment with 2057, we could not detect the expression of the surface antigens specific to the neutrophil cells, although enhanced cell motility was observed in HeLa cells. Our results suggest that 2057 activates Protein Kinase C, leading to the aberrant polymerization of the tubulin and delocalization of Lamin A/C, and induces the lobulation of the nuclei in HeLa cells.

Free Research Field

分子遺伝学