Coupling of spindle checkpoint with initiation of DNA replication

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650064
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: University of Hyogo
• Principal Investigator: Nishitani Hideo 兵庫県立大学, 生命理学研究科, 教授 (40253455)
• Co-Investigator(Renkei-kenkyūsha): SHIOMI Yasushi 兵庫県立大学, 大学院生命理学研究科, 准教授 (80380567) ; HAYASHI Akiyo 兵庫県立大学, 大学院生命理学研究科, 助教 (20779350)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 細胞周期 / 紡錘体 / DNA複製 / スピンドル / タンパク質分解

Outline of Final Research Achievements

Integrity of Genomic information during a cell cycle is maintained by precise replication and segregation of chromosomes. Cdt1, an essential factor acting for replication licensing, is degraded in S phase, but is stabilized upon entry into M phase. To prevent Cdt1 degradation, a mechanism operates that blocks binding of CyclinA-CDK to Cdt1. In M phase, Cdt1 was detected around the spindles proximal to the centrosome. In the absence of Cdt1, abnormal spindles were formed, spindle assembly checkpoint was activated, and anaphase onset was delayed. We propose that accumulation of Cdt1 in M phase couples initiation of S phase with mitotic events and ensures correct cell cycle progression.

Free Research Field

分子生物学