Identification of specific protein composes "fenestration" of glomerular endothelial cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650065
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Juntendo University
• Principal Investigator: ONDA Kisara 順天堂大学, 医学部, 非常勤助教 (60465044)
• Co-Investigator(Renkei-kenkyūsha): INOSHITA Hiroyuki 順天堂大学, 医学部, 准教授 (80646117)
• Research Collaborator: KUSABA Gaku 順天堂大学, 医学部
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 糸球体内皮細胞 / fenestration / 質量分析 / 新規蛋白質の同定 / カベオラ

Outline of Final Research Achievements

Renal dysfunction means declining of glomerular filtration which consists of glomerular basement membrane, podocyte slit diaphragm, and glomerular endothelial cell (GEnC) fenestrations. In this study, we aimed to identify specific proteins compose the fenestration. First, morphology of ultrastructure of human GEnC including fenestration was observed using electroscopy. To isolate GEnC from mice glomeruli, GEnC was cultured from Immortomouse glomeruli and purified by positive selection using anti-CD31 antibody. The selected GEnC was analyzed by FACS. Mass spectrometry should be done in next.

Free Research Field

腎臓内科学