Development of microfluidic cell culture system and analysis of stem cell differentiation from the perspective of spatiotemporal control of molecule

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: Maekawa Toshiro 東京大学, 生産技術研究所, 特任研究員 (30415294)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マイクロ流体デバイス / 幹細胞 / 時空間制御 / 細胞培養

Outline of Final Research Achievements

Cell culture method using conventional cell culture dishes, plates, just offers the uniform condition of cell culture environment. In this study, we developed a microfluidic cell culture system that enables spatiotemporal control for signal molecule concentration dynamically in cell culture chamber.
Evaluation for spatiotemporal control of molecule in the microfluidic device was performed using fluorescence dye as a visible substitute for molecule. In addition, it was confirmed that the microfluidic device is able to culture mouse iPS cells under the condition of spatiotemporal control of undifferentiation factors. These results indicate that this microfluidic cell culture system can be applied to investigation of mechanism in stem cell differentiation, and cellular response to dynamic change of signal molecule.

Free Research Field

分子細胞生物学