The elucidation of novel mechanisms of citrate synthase during early embryonic development

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Yamaguchi University
• Principal Investigator: IWAO Yasuhiro 山口大学, 医学(系)研究科(研究院), 教授 (10144916)
• Co-Investigator(Kenkyū-buntansha): UENO Shuichi 山口大学, 大学院医学系研究科, 准教授 (80363092)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: クエン酸合成酵素 / 初期発生 / 受精 / 細胞骨格 / 細胞分裂 / 微小管

Outline of Final Research Achievements

The role and localization of citrate synthase (CS) in cytoskeletons and ER were clarified from fertilization to early embryonic development. The changes in intracellular Ca2+ concentration was determined with fluorescent CS expressed in living fertilized eggs. The role of CS in the elongation of spindles during cleavage was investigated, and the localization of CS in the neural cells and muscle cells was confirmed. We succeeded to produce translucent blastomeres in the newt to establish the procedure for analyzing the role of CS during embryonic development.

Free Research Field

発生生物学