Establishment of transgenic reptiles by targeting spermatogonial stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650084
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Nomura Tadashi 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (10323007)
• Co-Investigator(Renkei-kenkyūsha): ONO Katsuhiko 京都府立医科大学, 教養部生物学教室・大学院神経発生生物学, 教授 (30152523) ; GOTOH Hitoshi 京都府立医科大学, 教養部生物学教室・大学院神経発生生物学, 助教 (20462202) ; MURAKAMI Yasunori 愛媛大学, 大学院理工学研究科, 准教授 (50342861)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: トランスジェニック動物 / 爬虫類 / 精巣 / 電気穿孔法 / 発現ベクター / トランスポゾン

Outline of Final Research Achievements

The aim of this project is the establishment of transgenic reptiles as a new experimental model of life sciences. To achieve this purpose, we targeted adult testicular stem cells and introduced expression vectors for fluorescent proteins (a linearized RFP expression vector and GFP expression vector with transposon sequences) into the testis of adult Madagascar ground geckoes. At 10 days after in vivo electroporation, we confirmed RFP and GFP expression in the testicular cells in seminiferous tubes. Furthermore, we detected RFP transgene in 1 of 8 embryos by genomic PCR analysis. These data suggest that making transgenic reptiles by targeting adult testicular stem cells is highly feasible, although further technical improvements are required to increase the efficiency of transgenesis.

Free Research Field

神経発生生物学