Role of a ribosomal protein in the ADAMTS protease pathway regulating cell migration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650086
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: Nishiwaki Kiyoji 関西学院大学, 理工学部, 教授 (30342827)
• Co-Investigator(Renkei-kenkyūsha): KIM Hon-Song 関西学院大学, 理工学研究科, 博士研究員 (70469899)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ADAMTSプロテアーゼ / リボソーム / 細胞移動 / 線虫

Outline of Final Research Achievements

MIG-17, an ADAMTS metalloprotease controls migration of gonadal distal tip cells (DTCs) through regulation of extracellular matrix in C. elegans. In the present study, we asked why the mutation in a ribosomal protein RPL-20(G82R) can suppress the DTC migration defect in the mig-17 mutants. Although we could not reach a clear understanding of the mechanism, we found that RPL-20(G82R) does not affect the regulatory mechanism of translational initiation. We also showed that RPL-20(G82R) is not secreted, but that it appears to act through unknown secreted proteins to suppress the mig-17 defects. We found that the RPL-20 activity is especially important in the intestine for the suppression to occur.

Free Research Field

発生生物学