Analysis of genetic factors involved in interaction between rhizobium and host plant through type III secretion system

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650089
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tohoku University
• Principal Investigator: Sato Shusei 東北大学, 生命科学研究科, 准教授 (70370921)
• Co-Investigator(Kenkyū-buntansha): KANEKO TAKAKAZU 京都産業大学, 総合生命科学部, 教授 (80370922)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 根粒菌-宿主相互作用 / TypeIII分泌系 / エフェクタータンパク質 / ミヤコグサ / 組換え近交系統 / ナチュラルバリエーション

Outline of Final Research Achievements

In an attempt to accumulate the information on the interaction between rhizobium and legume plants medicated by type III secretion system (T3SS) effectors, we conducted molecular genetic analyses using a model legume Lotus japonicus and Bradyrhizobium elkanii. As the results of infection analysis and Tn5 mutant screening, we identified that two types of immune responses, block of infection and prevention of nodule maturation, induced by different effectors. Based on the QTL analysis using RILs and GWAS using wild accessions of L. japonicus, we detected multiple candidate loci related to the responses caused by T3SS effectors.

Free Research Field

ゲノム構造機能学