Isolation of novel-type photosynthesis mutants of Chlamydomonas by phototaxis-based screening

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650093
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Wakabayashi Ken-ichi 東京工業大学, 科学技術創成研究院, 准教授 (80420248)
• Research Collaborator: Hisabori Toru 東京工業大学, 科学技術創成研究院, 教授 ; Ueki Noriko 東京工業大学, 科学技術創成研究院, 博士研究員 ; Ide Takahiro 東京工業大学, 資源化学研究所, 博士研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 走光性 / 光合成 / レドックス / 鞭毛

Outline of Final Research Achievements

For phototrophic microorganisms, it is important to inhabit under suitable light environment for phototaxis. Previously, we found that Chlamydomonas reinhardtii, a model organism to study photo-behavioral responses, shows positive phototaxis when cell is oxidized, and negative phototaxis when it is reduced. We came up with the idea to use this phenomenon as a new criterion for screening of novel-type photosynthesis mutants. In this research, we isolated three types of phototaxis-sign mutants, and one of them shows significantly high photosynthetic activity.

Free Research Field

細胞運動