2015 Fiscal Year Final Research Report
Analyses of nuclear fusion during plant reproduction using gametophyte cell-specific gene induction systems
| Project/Area Number |
26650094
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Research Collaborator |
KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授
URAWA Hiroko 岐阜聖徳学園大学, 教育学部, 准教授
YAMAGUCHI Yuki 新潟大学, 大学院自然科学研究科, 大学院生
WADA Satomi 新潟大学, 大学院自然科学研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 核膜融合 / 有性生殖 / 遺伝子発現誘導系 / シロイヌナズナ / 配偶体 |
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| Outline of Final Research Achievements |
In this study, we developed a new gene induction method, which allows cell-specific gene induction in Arabidopsis female gametophytes using heat shock-induced Cre-loxP recombination and female gametophyte-specific promoters. Female gametophyte-specific gene expressions were induced by heat treatment of flower buds of constructed transgenic lines. We also showed induction of cell-specific gene expression by irradiating isolated female gametophytes with the infrared laser. The developed method will be a good tool to analyze functions of proteins involved in the fusion of polar nuclei during female gametophyte development.
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| Free Research Field |
植物細胞生物学
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