2016 Fiscal Year Final Research Report
Development of an inducible gene expression system with a plant UV-B receptor and pulse laser
| Project/Area Number |
26650096
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 転写誘導系 / 紫外線 / シロイヌナズナ / UVレーザー / UV-B受容体 |
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| Outline of Final Research Achievements |
The Arabidopsis UV-B receptor UVR8 and UV pulse laser are utilized for the purpose of developing an artificial induction system of gene expression with a high spatiotemporal resolution. UVR8 forms a homodimer in the nucleus under normal plant growth conditions, and reversibly dissociates to interact with other regulatory proteins in response to UV-B irradiation. Taking advantage of these characteristics of UVR8, construction of an UV-inducible transcription system was attempted by combining molecular functions of LexA, the transcription activation domain of VP16, the SRDX motif, and the WD40 domain of COP1.
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| Free Research Field |
植物分子生物学
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