2017 Fiscal Year Final Research Report
Development of high-resolution three-dimensional fluorescence imaging method using vector beams of excitation laser
| Project/Area Number |
26650107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Ehime University (2017)
Hokkaido University (2014-2016) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 集光特性 / 固定脳 / 生体脳 / 超解像顕微鏡 |
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| Outline of Final Research Achievements |
Evaluation of the effect of vector beam in fixed brain slice implanted fluorescent beads suggests that it shows different optical characteristics and focusing properties even in linearly polarized light. Next, Evaluating the optical properties in the fixed brain with a novel clearing reagent, conditions of that were maintained at a depth of several micrometer from the surface. Therefore, we tried to be applied the methods to super-resolution microscope (structured illumination microscope), the effect was obtained in the deep region and the spine structure of neuronal synapse in more detail which was not normally observable. Next, we tried to analyze the optical properties in the living brain, and found some conditions by adjusting the angle of the cover glass and the refractive index of the immersion liquid.
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| Free Research Field |
顕微鏡
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