Circumferential asymmetry of mouse nodal cilia

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650112
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: National Institute for Basic Biology
• Principal Investigator: Nonaka Shigenori 基礎生物学研究所, 時空間制御研究室, 准教授 (90435529)
• Research Collaborator: TANIGUCHI Atsushi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 繊毛 / 左右性 / 発生生物学

Outline of Final Research Achievements

In mammalian development, leftward fluid flow generated by nodal cilia is crucial to future left-right asymmetry. The cilia beat in rotational pattern with different angular velocity between leftward and rightward phases, and the cause of the difference is not determined, manifestation of viscous resistance or active control based on the ciliary structure. That’s why we tried to find out circumferential orientation of the cilia, by determining basal foot and mother/daughter centrioles at the base of the cilia. Unfortunately, the reported marker of basal foot, ODF2, exhibited unexpected staining pattern and we couldn’t determine their exact orientation, while the orientation of the two centrioles (mother or daughter) seemed random, suggesting the viscous resistance model.

Free Research Field

発生生物学