Analysis of a novel role of an RNA splicing factor in somatic hypermutation of the IgV gene

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650126
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Okayama University
• Principal Investigator: Kanayama Naoki 岡山大学, 自然科学研究科, 准教授 (70304334)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 抗体 / 体細胞高頻度突然変異 / 親和性成熟 / スプライシング因子 / DT40

Outline of Final Research Achievements

In this study, we analyzed molecular mechanisms for SRSF1-3 to contribute to somatic hypermutation of the IgV gene. It is revealed that although isoforms SRSF1 and SRSF1-3 are different only in the C-terminal region, the C-terminal region of SRSF1-3 is not essential for SHM, suggesting that the conserved region might be involved in SHM. In addition, this study indicated that SRSF1-3 may have a role in regulating the nuclear export of AID during SHM processes.

Free Research Field

細胞工学、免疫学