High-throughput DNA barcoding for foodweb analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 26650144
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Kyoto University
• Principal Investigator: Toju Hirokazu 京都大学, 人間・環境学研究科(研究院), 助教 (60585024)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 生態学 / 群集 / 食物網 / ネットワーク / DNAバーコーディング

Outline of Final Research Achievements

In this research project, I developed a pipeline for detecting prey DNA in predator samples through high-throughput DNA barcoding based on next-generation sequencing. The pipeline consists of improved methods of sampling, DNA extraction, PCR, next-generation sequencing, bioinformatics, and community ecological statistical analyses. Because the pipeline is based on the universal DNA barcoding system (http://www.claident.org/), it is applicable to various types of interspecific interaction systems (e.g., food webs) involving various taxonomic groups of organisms.

Free Research Field

生態学