Mitochondrial genome complete sequencing and technological development of computer scientific "cell fractionation method" in fern

2016 Fiscal Year Final Research Report

• Project/Area Number: 26650146
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Women's University
• Principal Investigator: Ootsuki Ryo 日本女子大学, 理学部, 研究員 (10646962)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 次世代シーケンス / シダ植物 / ミトコンドリアゲノム / 無配生殖

Outline of Final Research Achievements

In this study, we succeeded in determining the full length sequence of chloroplast of Cyrtomium fortunei by Next Generation Sequencing data (NGS). In mitochondrial genomes, we were able to determine the full length sequence of the major genes. But, the complete mitochondrial genome sequence which containing all the intergenic regions as originally planned could not be determined. Because, there may be multiple structural polymorphism (multipartite structure) in the mitochondrial genome.

Free Research Field

植物系統分類学