Screening of novel active retrotransposon families with a potential for accelerating the genetic analysis in polyploid species

2015 Fiscal Year Final Research Report

• Project/Area Number: 26660007
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Okayama University
• Principal Investigator: Monden Yuki 岡山大学, 環境生命科学研究科, 助教 (30646089)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 倍数性 / 遺伝解析 / 高速シーケンサー / 転移因子

Outline of Final Research Achievements

The aim of this research was to screen the retrotransposon families that showed high insertion polymorphisms among crop cultivars. It was shown that the insertion polymorphisms of those families were quite useful for the genetic analysis in polyploidy species. A novel method was developed by modifying a previous method, which could be applied to non-model crop species including sweetpotato. As a result, 14 new retrotransposon families were identified in sweetpotato, and we experimentally verified their insertion polymorphisms. In addition, one retrotransposon family was cloned and its entire sequence could be determined. Moreover, comprehensive analyses of the insertion sites for several families were conducted. Thus, we achieved our initial goal.

Free Research Field

農学